Structural conservation of RecF and Rad50: implications for DNA recognition and RecF function
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چکیده
منابع مشابه
Structural conservation of RecF and Rad50: implications for DNA recognition and RecF function.
RecF, together with RecO and RecR, belongs to a ubiquitous group of recombination mediators (RMs) that includes eukaryotic proteins such as Rad52 and BRCA2. RMs help maintain genome stability in the presence of DNA damage by loading RecA-like recombinases and displacing single-stranded DNA-binding proteins. Here, we present the crystal structure of RecF from Deinococcus radiodurans. RecF exhibi...
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Homologous, or general, recombination is a crucial biological process that involves the paring and transfer of strands between DNA molecules that share a region of significant sequence homology. After its discovery in 1946 by Lederberg and Tatum (48), homologous recombination in bacteria was associated with the sexual process of conjugation and was viewed as an evolutionary mechanism both for s...
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The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR ...
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In all cells, genetic recombination is used to repair DNA breaks and, as a result, genetic information is exchanged between homologous DNA molecules. Discontinuities in DNA strands, specifically double-strand DNA breaks and single-strand DNA gaps, attract the enzymes responsible for the initiation of homologous recombination. In wild-type Escherichia coli, two distinct pathways are responsible ...
متن کاملrecF-dependent and recF recB-independent DNA gap-filling repair processes transfer dimer-containing parental strands to daughter strands in Escherichia coli K-12 uvrB.
The processes for repairing DNA daughter-strand gaps were studied in UV-irradiated uvrB, uvrB recB, uvrB recF, and uvrB recB recF cells of Escherichia coli K-12. The dimer-containing parental DNA was found to be joined to daughter strands during postreplication repair in all four strains examined. Therefore, both the major (recF-dependent) and the minor (recF recB-independent) gap-filling proce...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2007
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7601537